PBMC Isolation
Analysing peripheral blood mononuclear cells (PBMCs) offers a comprehensive assessment of the immune system’s response to treatment, highlighting their crucial role in clinical trial sample analysis. The accuracy and reliability of data generated from assays that utilise PBMCs hinge on the prompt collection, skilled isolation, handling, and storage of samples.
Expertise With
PBMC Isolation:
Experienced in Various Isolation Techniques
Our expertise includes transitional density gradient underlay, SepMate™, LeucoSep™, and CPT™. Our extensive SOP list has over 50 custom methods.
Quality Assurance
Accreditation by Australia’s National Association of Testing Authorities (NATA) and participation in external and internal proficiency programs ensures consistent, high-quality isolation of PBMCs.
Flexibility
Our onsite phlebotomists ensure we can provide donor whole blood for study-specific proficiency testing, and our comprehensive immunology profiling services allow for downstream assay support or shipment of PBMCs to a client’s selected laboratory.
360biolabs Central Lab Services
As part of 360biolabs Central Laboratory Services, we offer PBMC isolation and RNA/DNA, with the added capability to handle more complex matrices and coordinate global shipping to any preferred laboratory.
PBMC Isolation
- Study specific proficiency testing
- Temperature monitored shipments
- Onsite 24/7 temperature monitoring
- GLP, GCP, ISO17025 accredited laboratories
- Time sensitive isolation via our multiple sites
- Experienced in isolating PBMCs from:
- ACD
- EDTA
- Sodium Heparin
- Lithium Heparin
- CPT
RNA/DNA Extraction
- Extraction of high-quality RNA and DNA
- Small scale extraction using Spin Column technology: Mini, Midi & Maxi
- Automated extraction process: Magnetic Bead based extraction system
- Matrices we support:
- Whole blood
- Serum (Circulating Tumor DNA)
- Plasma
- Stool
- Urine
- Oral mucosall swab
- Injection site swab
- Biopsies (Fresh Frozen or FFPE fixed)
- Cell Culture supernatant
- Nasal mucosal lining fluid
- Saliva
- Synovial fluid
- Whole blood
- CSF
- Wound fluid
Specialised Matrix
- Processing of specialised matrices:
- Bone marrow aspirate
- Sputum
Related Content
ELISpot
ELISpot measures the frequency of antigen-specific cells by measuring the number of cytokine-secreting cells in PBMC at a single cell level.
Flow Cytometry
Using high-parameter single-cell analysis, flow cytometry can identify granular changes in response to a therapeutic.
Molecular Assays
Using high-parameter single-cell analysis, flow cytometry can identify granular changes in response to a therapeutic.